Analysis of the heterogeneity of the BCR H-CDR3 repertoire in the bone marrow and spleen of 3-, 12-, and 20-month old mice

Abstract The number of central and peripheral B cells their responsiveness are decreased in aged mice. diversity mice cell repertoires with increasing age has not been elucidated. In this study, we demonstrated that there were significant differences the usage some V , D J genes BCR H-CDR3 repertoire bone marrow cells, spleen memory 3-, 12-, 20-month-old productive, pseudogene, out-of-frame sequences, had 5′J trimming age; N1 insertion, N2 P5’D 5’D age. proportion overlap but at different ages was lower 3 months than 12 20 months. This study is first to report homogeneity heterogeneity CDR3 change as age, further investigation decline response immunity young/middle/old-aged